Author: Charles L Howe; Reghann G. LaFrance-Corey; Emma N Goddery; Kanish Mirchia
Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection Document date: 2017_10_25
ID: ebqquj7i_11
Snippet: Following TMEV infection, serum and whole brain or hippocampal homogenates were collected, clarified, and stored at -80°C until analysis. Mouse CCL2 was detected using the Quantikine ELISA kit (R&D Systems, Minneapolis, MN) following the manufacturer's instructions. For each hippocampus and whole brain sample, 50 µL of neat homogenate was measured in duplicate. Serum samples were diluted 2-fold and measured in duplicate. Values were calculated .....
Document: Following TMEV infection, serum and whole brain or hippocampal homogenates were collected, clarified, and stored at -80°C until analysis. Mouse CCL2 was detected using the Quantikine ELISA kit (R&D Systems, Minneapolis, MN) following the manufacturer's instructions. For each hippocampus and whole brain sample, 50 µL of neat homogenate was measured in duplicate. Serum samples were diluted 2-fold and measured in duplicate. Values were calculated from a standard curve included in every assay. Serum values are reported as per mL; tissue homogenate values were back-calculated to the total amount present in the entire animal.
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