Selected article for: "pcr machine and real time"
Author: Mehdi Baratchian; Jeff McManus; Mike Berk; Fumihiko Nakamura; Serpil Erzurum; Sanjay Mukhopadhyay; Judy Drazba; John Peterson; Ben Gaston; Nima Sharifi
Title: No evidence that androgen regulation of pulmonary TMPRSS2 explains sex-discordant COVID-19 outcomes
  • Document date: 2020_4_21
    • ID: awb1stsr_36
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.21.051201 doi: bioRxiv preprint Total RNA was harvested by homogenizing 25 mg lung tissue in 350 µl RLT buffer (RNeasy kit, Qiagen) following the manufacturer's instructions. cDNA synthesis were then carried out with the iScript cDNA Synthesis Kit (Bio-Rad). Quantitative PCR (qPCR) analysis was conducted in triplicate in an ABI 7500 Real.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.21.051201 doi: bioRxiv preprint Total RNA was harvested by homogenizing 25 mg lung tissue in 350 µl RLT buffer (RNeasy kit, Qiagen) following the manufacturer's instructions. cDNA synthesis were then carried out with the iScript cDNA Synthesis Kit (Bio-Rad). Quantitative PCR (qPCR) analysis was conducted in triplicate in an ABI 7500 Real-Time PCR machine (Applied Biosystems) using iTaq Fast SYBR Green Supermix with ROX (Bio-Rad) with the following primer sets:

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