Selected article for: "lysis buffer and macherey nagel nucleospin rna"
Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA
  • Document date: 2020_3_29
    • ID: nil1vv6h_27
    Snippet: Leeches were cut into small pieces with a new scalpel blade and lysed overnight (≥12 hours) at 55°C in proteinase K and ATL buffer at a ratio of 1:10; 0. Water and sediment 500 µl of the centrifuged filtrate was used to extract viral nucleic acids using the RTP ® DNA/RNA Virus Mini Kit (Stratec biomedical). The following modifications were made to the original protocol: 400 µl of lysis buffer, 400 µl of binding buffer and 20 µl of protein.....
    Document: Leeches were cut into small pieces with a new scalpel blade and lysed overnight (≥12 hours) at 55°C in proteinase K and ATL buffer at a ratio of 1:10; 0. Water and sediment 500 µl of the centrifuged filtrate was used to extract viral nucleic acids using the RTP ® DNA/RNA Virus Mini Kit (Stratec biomedical). The following modifications were made to the original protocol: 400 µl of lysis buffer, 400 µl of binding buffer and 20 µl of proteinase K and carrier RNA were used per sample. Samples were eluted in 60 µl. The NucleoSpin Soil kit (Macherey-Nagel) was used to extract DNA/RNA from sediment. 500 mg of soil was extracted according to the manufactures protocol using an elution volume of 100 µl.

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