Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow Document date: 2020_3_10
ID: kv77pw7y_9
Snippet: Each viral primer/probe set was multiplexed with primer/probe set for human RPP30 gene to control the sample collection, as the amount of biological material varies from sample to sample, and with a primer/probe set for an artificial RNA sequence (artificial external control -AEC), which is introduced during extraction, to control the RT-qPCR steps and its inhibition. Primers and probes for RPP30 and AEC can be found in the supplemental file......
Document: Each viral primer/probe set was multiplexed with primer/probe set for human RPP30 gene to control the sample collection, as the amount of biological material varies from sample to sample, and with a primer/probe set for an artificial RNA sequence (artificial external control -AEC), which is introduced during extraction, to control the RT-qPCR steps and its inhibition. Primers and probes for RPP30 and AEC can be found in the supplemental file.
Search related documents:
Co phrase search for related documents- biological material and sample collection: 1
- inhibition RT qPCR step and RT qPCR step: 1, 2
- primer probe and probe primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89
- primer probe and RNA sequence: 1, 2, 3
- primer probe and RT qPCR step: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- primer probe and viral primer probe set: 1
- primer probe set and probe primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62
- primer probe set and RT qPCR step: 1, 2
- primer probe set and viral primer probe set: 1
- probe primer and RNA sequence: 1, 2, 3
- probe primer and RT qPCR step: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- probe primer and viral primer probe set: 1
Co phrase search for related documents, hyperlinks ordered by date