Selected article for: "primer probe and RT qPCR"
Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow
  • Document date: 2020_3_10
    • ID: kv77pw7y_9
    Snippet: Each viral primer/probe set was multiplexed with primer/probe set for human RPP30 gene to control the sample collection, as the amount of biological material varies from sample to sample, and with a primer/probe set for an artificial RNA sequence (artificial external control -AEC), which is introduced during extraction, to control the RT-qPCR steps and its inhibition. Primers and probes for RPP30 and AEC can be found in the supplemental file......
    Document: Each viral primer/probe set was multiplexed with primer/probe set for human RPP30 gene to control the sample collection, as the amount of biological material varies from sample to sample, and with a primer/probe set for an artificial RNA sequence (artificial external control -AEC), which is introduced during extraction, to control the RT-qPCR steps and its inhibition. Primers and probes for RPP30 and AEC can be found in the supplemental file.

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