Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA Document date: 2020_3_29
ID: nil1vv6h_3
Snippet: In order to test the sensitivity of the viral capture in recovering vertebrate host viruses, the capture system was first applied to a positive control consisting of medical leeches fed with human blood spiked with two RNA viruses and two DNA viruses at different concentrations [8] . All four viruses were detected, even if enrichment efficiency (proportion of on-target viral reads) and target genome recovery varied among viruses (Suppl. Fig. 1) ......
Document: In order to test the sensitivity of the viral capture in recovering vertebrate host viruses, the capture system was first applied to a positive control consisting of medical leeches fed with human blood spiked with two RNA viruses and two DNA viruses at different concentrations [8] . All four viruses were detected, even if enrichment efficiency (proportion of on-target viral reads) and target genome recovery varied among viruses (Suppl. Fig. 1) . No viral contigs were identified in the negative controls included to monitor laboratory contaminations for either the leech or water experiments.
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