Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA Document date: 2020_3_29
ID: nil1vv6h_38
Snippet: A total of 219,580,903 sequence reads 300 bp long were generated (average: 3,181,781 single reads per sample; standard deviation [SD]: 1,481,098) (Suppl. Tab. 1) and sorted by their dual index sequences. Cutadapt v1.16 and Trimmomatic v0.36 were used to remove adapter sequences and low-quality reads using a quality cutoff of 20 and a minimal read length of 30 nt. After trimming, 97% of the sequences were retained. Three different approaches (A, B.....
Document: A total of 219,580,903 sequence reads 300 bp long were generated (average: 3,181,781 single reads per sample; standard deviation [SD]: 1,481,098) (Suppl. Tab. 1) and sorted by their dual index sequences. Cutadapt v1.16 and Trimmomatic v0.36 were used to remove adapter sequences and low-quality reads using a quality cutoff of 20 and a minimal read length of 30 nt. After trimming, 97% of the sequences were retained. Three different approaches (A, B, C) were used to analyse the viral capture data:
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