Author: Ali Punjani; Haowei Zhang; David J. Fleet
Title: Non-uniform refinement: Adaptive regularization improves single particle cryo-EM reconstruction Document date: 2019_12_16
ID: bqwmx5dy_41
Snippet: For PfCRT, the difference in resolution and map quality between uniform and non-uniform refinement is dramatic. Figure 4A ,C shows the corresponding FSC curves and 3D density maps. Uniform refinement is limited to 6.9Ã…, a resolution at which transmembrane helices are barely resolvable. At this low resolution, the authors [15] were unable to interpret the density map nor improve it with conventional techniques. By contrast, their results and ours.....
Document: For PfCRT, the difference in resolution and map quality between uniform and non-uniform refinement is dramatic. Figure 4A ,C shows the corresponding FSC curves and 3D density maps. Uniform refinement is limited to 6.9Ã…, a resolution at which transmembrane helices are barely resolvable. At this low resolution, the authors [15] were unable to interpret the density map nor improve it with conventional techniques. By contrast, their results and ours here show that non-uniform refinement recovers signal up to 3.6Ã…, from which an atomic model can be built with confidence. Such levels of improvement are the difference between success and failure in a cryo-EM project.
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