Selected article for: "DNA RNA Nucleic acid and Nucleic acid"
Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods
  • Document date: 2020_4_5
    • ID: ec3egn8o_19
    Snippet: Synthetic nucleic acid controls (Integrated DNA Technologies) and extracted RNA from a SARS-CoV-2 clinical isolate (USA-WA1/2020) were also used as direct PCR inputs to demonstrate specificity of the assay. The clinical isolate and plasmid nCov2 controls were both positive for the N1 and N2 reactions and negative for RP, as expected. SARS and MERS nucleic acid specificity controls (run in triplicate) were negative for all three targets, also as e.....
    Document: Synthetic nucleic acid controls (Integrated DNA Technologies) and extracted RNA from a SARS-CoV-2 clinical isolate (USA-WA1/2020) were also used as direct PCR inputs to demonstrate specificity of the assay. The clinical isolate and plasmid nCov2 controls were both positive for the N1 and N2 reactions and negative for RP, as expected. SARS and MERS nucleic acid specificity controls (run in triplicate) were negative for all three targets, also as expected (Table 3 ).

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