Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_27
Snippet: Analytical Precision Reproducibility of results between RNA replicates of both clinical samples and synthetic or genomic isolate controls was characterized across these validation experiments. We tested a total of 24 replicate sample pairs, not including the limit of detection replicates described above. Comparing across separate PCR plates (inter-run), we had complete concordance between 4 clinical sample pairs and 6 control sample pairs (Table .....
Document: Analytical Precision Reproducibility of results between RNA replicates of both clinical samples and synthetic or genomic isolate controls was characterized across these validation experiments. We tested a total of 24 replicate sample pairs, not including the limit of detection replicates described above. Comparing across separate PCR plates (inter-run), we had complete concordance between 4 clinical sample pairs and 6 control sample pairs (Table 9 ). Within single PCR plates (intra-run), we showed 100% concordance between 6 clinical sample and 8 control sample pairs.
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