Author: Dupas Enora; Briand Martial; Jacques Marie-Agnès; Cesbron Sophie
Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues Document date: 2019_7_11
ID: 65qpcsiq_42
Snippet: The LOD of Xf in V. vinifera was 100 times higher in the second assay highlighting a potential 332 accumulation of qPCR inhibitors between the two experiments. Moreover, on 11 combinations out of 333 46, XF primers had a LOD 10 times higher in planta than the one obtained for the subspecies. Xf 334 subspecies could be identified until a Ct value of 35.08 using Harper's qPCR assay in a spiked sample 335 . CC-BY-NC-ND 4.0 International license is m.....
Document: The LOD of Xf in V. vinifera was 100 times higher in the second assay highlighting a potential 332 accumulation of qPCR inhibitors between the two experiments. Moreover, on 11 combinations out of 333 46, XF primers had a LOD 10 times higher in planta than the one obtained for the subspecies. Xf 334 subspecies could be identified until a Ct value of 35.08 using Harper's qPCR assay in a spiked sample 335 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/699371 doi: bioRxiv preprint in planta identification of Xylella fastidiosa subspecies 9 of P. dulcis. In other matrices the LOD of the tetraplex qPCR assay corresponded usually to a Ct value 336 ranging from 30 to 34 using Harper's qPCR. 337
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