Author: Mandy Muller; Britt A. Glaunsinger
Title: Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases Document date: 2017_6_26
ID: jhwjh12k_25
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/155457 doi: bioRxiv preprint Depletion of either HuR or NCL impairs the ability of the IL-6 derived SRE to protect 305 its associated mRNA from degradation by SOX [26, 27] . Given that both proteins are also 306 bound by the G-SRE, we evaluated whether they were similarly important for G-SRE-307 mediated escape. We individually dep.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/155457 doi: bioRxiv preprint Depletion of either HuR or NCL impairs the ability of the IL-6 derived SRE to protect 305 its associated mRNA from degradation by SOX [26, 27] . Given that both proteins are also 306 bound by the G-SRE, we evaluated whether they were similarly important for G-SRE-307 mediated escape. We individually depleted each protein from 293T cells using siRNAs 308 targeting HuR or NCL (or control non-targeting siRNAs), then measured the ability of SOX 309 to degrade the GFP-GADD45B-3'UTR reporter by RT-qPCR ( Fig. 6A & 6B) . Similar to the IL-310 6 SRE, depletion of HuR eliminated the protective capacity of the G-SRE, leading to 311 degradation of the GFP-GADD45B-3'UTR mRNA in SOX-expressing cells (Fig. 6A) . 312
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