Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases

Mandy Muller; Britt A. Glaunsinger

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/155457 doi: bioRxiv preprint of that gene is broadly required for infection. Finally, it is important to bear in mind that 397 the SREs discovered thus far are cellular elements that assemble cellular proteins-and 398 thus presumably play host-directed roles in the regulation of their associated transcripts. It 399 may therefore be the case that some SRE-bearing mRNAs function in an antiviral capacity. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. centrifugation at (14,000rpm for 10 min). Two mL of hybridization buffer was added to 454 each sample along with 100pmol of the indicated probes (see Table S3 for sequences). were added for 1h, washed three times with lysis buffer and twice with high-salt lysis 487 buffer (low-salt lysis buffer except containing 400mM NaCl). Samples were separated into 488 All rights reserved. No reuse allowed without permission.