Selected article for: "real time and thermal cycling"

Author: Liwen Chen; Chaohua Deng; Xuhui Chen; Xian Zhang; Bo Chen; Huimin Yu; Yuanjun Qin; Ke Xiao; Hong Zhang; Xufang Sun
Title: Ocular manifestations and clinical characteristics of 534 cases of COVID-19 in China: A cross-sectional study
  • Document date: 2020_3_16
  • ID: 5zrywg58_9
    Snippet: Nasopharyngeal swabs were collected from all patients by a trained healthcare worker using protective equipment. The swab samples were maintained in a viral-transport medium stored between 2° C and 8° C and were analyzed by real-time RT-PCR. Thermal cycling was performed at 55° C for 10 min for reverse transcription, followed by 95° C for 3 min and then 45 cycles of 95° C for 15 s and 58° C for 30 s......
    Document: Nasopharyngeal swabs were collected from all patients by a trained healthcare worker using protective equipment. The swab samples were maintained in a viral-transport medium stored between 2° C and 8° C and were analyzed by real-time RT-PCR. Thermal cycling was performed at 55° C for 10 min for reverse transcription, followed by 95° C for 3 min and then 45 cycles of 95° C for 15 s and 58° C for 30 s.

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