Selected article for: "extraction kit and QE buffer"
Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV
  • Document date: 2020_4_5
    • ID: ihhu7nef_10
    Snippet: In the parallel comparison of QE buffer lysed samples versus column purified samples, the Ct values for positive controls and RNaseP were within 1 cycle or less, even though the samples were concentrated 4x (from 120 ul to 30 ul elute). It indicates that there is significant loss of material by column purification and the benefit of concentrating the samples is evened out. Additionally, our method starts with only 200 ul of lysis buffer, and 2 ul.....
    Document: In the parallel comparison of QE buffer lysed samples versus column purified samples, the Ct values for positive controls and RNaseP were within 1 cycle or less, even though the samples were concentrated 4x (from 120 ul to 30 ul elute). It indicates that there is significant loss of material by column purification and the benefit of concentrating the samples is evened out. Additionally, our method starts with only 200 ul of lysis buffer, and 2 ul of direct lysis is 1/100 of the total sample. If we use a smaller swab, which is available, the volume can be lowered further. With the standard method, the sample transport buffer is 3 mL and only 120 ul to 140 ul, depending on the extraction kit, is taken for purification, essentially a 1:15 dilution that can translate into up to a difference of 4 Ct's.

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