Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_29
Snippet: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022384 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022384 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . Tabl.....
Document: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022384 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022384 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . Table 1 Comparison of QE lysis performance across specimen storage temperatures after 24 hours. Ct values for oropharyngeal (OP) and nasopharyngeal (NP) specimens that were immediately heat extracted (no storage) or kept at room temperature (RT), 4°C or -20°C for 24 hours. The presence of the collection swab during heat extraction is also shown (+/-swab). The positive control is plasmid DNA template spiked at 100 copies/ul into specimen sample. A value of NS indicates no signal was detected.
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