Author: Jing-Wen Ai; Hao-Cheng Zhang; Teng Xu; Jing Wu; Mengqi Zhu; Yi-Qi Yu; Han-Yue Zhang; Zhongliang Shen; Yang Li; Xian Zhou; Guo-Qing Zang; Jie Xu; Wen-Jing Chen; Yong-Jun Li; De-Sheng Xie; Ming-Zhe Zhou; Jing-Ying Sun; Jia-Zhen Chen; Wen-Hong Zhang
Title: Optimizing diagnostic strategy for novel coronavirus pneumonia, a multi-center study in Eastern China Document date: 2020_2_17
ID: nqxo3f45_10
Snippet: At the meantime, 300μl of UTM were sent for a fast, simultaneous, multiplexed real-time reverse transcription nucleic acid amplification assay for qualitative detection and identification of multiple respiratory viral and bacterial nucleic acids in nasopharyngeal swabs using Qiagen ResPlex II V2.0 kit, which including more than 22 Total RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Germany), and sent for the metagenomic sequenci.....
Document: At the meantime, 300μl of UTM were sent for a fast, simultaneous, multiplexed real-time reverse transcription nucleic acid amplification assay for qualitative detection and identification of multiple respiratory viral and bacterial nucleic acids in nasopharyngeal swabs using Qiagen ResPlex II V2.0 kit, which including more than 22 Total RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Germany), and sent for the metagenomic sequencing. During metagenomic sequencing, RNA was reversetranscribed into cDNA, with which sequencing library was prepared. The qualified library was All rights reserved. No reuse allowed without permission. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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