Author: Aditi Joshi; Sushmita Paul
Title: Phylogenetic Analysis of the Novel Coronavirus Reveals Important Variants in Indian Strains Document date: 2020_4_15
ID: eyi5609q_6
Snippet: SRA project sequences were aligned to human genome assembly (hg19) using 41 HiSat2 [10] , to remove the human contamination in viral sequences. Next the unaligned 42 region to hg19 was aligned to SARS-CoV2 ref sequence (NC 045512) using 43 SamTools [13] and HiSat2. Consensus fasta sequence of four viral strains of project were 44 obtained using BcfTools [13] . with Tamura-Nei model and Neighbour-Joining method along with 100 bootstrap steps 52 us.....
Document: SRA project sequences were aligned to human genome assembly (hg19) using 41 HiSat2 [10] , to remove the human contamination in viral sequences. Next the unaligned 42 region to hg19 was aligned to SARS-CoV2 ref sequence (NC 045512) using 43 SamTools [13] and HiSat2. Consensus fasta sequence of four viral strains of project were 44 obtained using BcfTools [13] . with Tamura-Nei model and Neighbour-Joining method along with 100 bootstrap steps 52 using the MEGAX.
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