Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_16
Snippet: • Binding buffer: Mix 200 μL 1M HEPES-KOH pH 7.9, 100 μL 1M KCl, 10 μL 1M CaCl 2 and 10 μL 1M MnCl 2 , and bring the final volume to 10 mL with dH 2 O. Store the buffer at 4°C for up to several months. • Wash buffer: Mix 1 mL 1 M HEPES pH 7.5, 1.5 mL 5 M NaCl, 12.5 μL 2 M spermidine, bring the final volume to 50 mL with dH 2 O, and add 1 Roche Complete Protease Inhibitor EDTA-Free tablet. Store the buffer at 4°C for up to several month.....
Document: • Binding buffer: Mix 200 μL 1M HEPES-KOH pH 7.9, 100 μL 1M KCl, 10 μL 1M CaCl 2 and 10 μL 1M MnCl 2 , and bring the final volume to 10 mL with dH 2 O. Store the buffer at 4°C for up to several months. • Wash buffer: Mix 1 mL 1 M HEPES pH 7.5, 1.5 mL 5 M NaCl, 12.5 μL 2 M spermidine, bring the final volume to 50 mL with dH 2 O, and add 1 Roche Complete Protease Inhibitor EDTA-Free tablet. Store the buffer at 4°C for up to several months.
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