Author: Yafei Wang; Randy Heiland; Morgan Craig; Courtney L. Davis; Ashlee N Ford Versypt; Adrianne Jenner; Jonathan Ozik; Nicholson Collier; Chase Cockrell; Andrew Becker; Gary An; James A. Glazier; Aarthi Narayanan; Amber M Smith; Paul Macklin
Title: Rapid community-driven development of a SARS-CoV-2 tissue simulator Document date: 2020_4_5
ID: lq4tcyh4_76
Snippet: From a host response point of view, we will pursue two aspects: host cell death and inflammatory responses. For cell survival and death measurements, we will employ an assay that measures ATP activity in cells (hence a reflection of a live cell) in the context of infection and inhibitor treatments. For inflammatory responses, we will assess supernatants for inflammatory mediators by ELISA (multiplexed). The cells will be lysed to obtain RNA, whic.....
Document: From a host response point of view, we will pursue two aspects: host cell death and inflammatory responses. For cell survival and death measurements, we will employ an assay that measures ATP activity in cells (hence a reflection of a live cell) in the context of infection and inhibitor treatments. For inflammatory responses, we will assess supernatants for inflammatory mediators by ELISA (multiplexed). The cells will be lysed to obtain RNA, which will be queried for transcription of several genes associated with inflammatory responses using gene expression arrays (multiplexed).
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