Selected article for: "gene sequence and nucleocapsid gene"
Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler
  • Document date: 2020_4_20
    • ID: e1oinu71_6
    Snippet: Samples and controls used for assay development For biosafety purposes, synthetic nucleic acids rather than whole virus templates were used for the development of this assay. As SARS-CoV-2 is a positive stranded RNA coronavirus, we synthesized single stranded RNA (ssRNA) of the amplicon sequence (99 bp) for SARS-CoV-2 nucleocapsid (N) gene (Integrated DNA Technologies, San Diego). Plasmid DNA containing the N gene of SARS-CoV-2, MERS-CoV and SARS.....
    Document: Samples and controls used for assay development For biosafety purposes, synthetic nucleic acids rather than whole virus templates were used for the development of this assay. As SARS-CoV-2 is a positive stranded RNA coronavirus, we synthesized single stranded RNA (ssRNA) of the amplicon sequence (99 bp) for SARS-CoV-2 nucleocapsid (N) gene (Integrated DNA Technologies, San Diego). Plasmid DNA containing the N gene of SARS-CoV-2, MERS-CoV and SARS-CoV were also used as positive controls (2019-nCoV RUO Plasmid Controls, Integrated DNA Technologies, San Diego). ssRNA of human ribonuclease P (RP) gene amplicon sequence (65 bp) was synthesized for use as internal control (Integrated DNA Technologies, San Diego).

    Search related documents:
    Co phrase search for related documents
    • amplicon sequence and plasmid dna: 1
    • amplicon sequence and RNA coronavirus: 1, 2
    • assay development and control sample: 1
    • assay development and internal control: 1
    • assay development and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • assay development and plasmid dna: 1
    • assay development and positive control: 1
    • assay development and RNA coronavirus: 1, 2
    • assay development and SARS nucleocapsid: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • control sample and internal control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • control sample and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • control sample and plasmid dna: 1, 2, 3, 4
    • control sample and positive control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • control sample and RNA coronavirus: 1
    • control sample and SARS CoV MERS CoV SARS: 1
    • control sample and SARS nucleocapsid: 1, 2
    • internal control and RP gene: 1, 2, 3, 4, 5
    • internal control and SARS CoV MERS CoV SARS: 1
    • internal control and SARS nucleocapsid: 1, 2, 3, 4, 5