Selected article for: "PCR assay and qpcr PCR"
Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples
  • Document date: 2019_5_10
    • ID: hxmk6gvr_41
    Snippet: Sequencing data was further analyzed to determine if multiple IBV genotypes or lineages could be detected. Lineage-1 and lineage-27 from Genotype I (GI-L1, GI-L27), and lineage-1 from Genotype IV (GIV-L1) were the detected lineages in the samples. Results from individual RT-qPCR assay for GA08 (GI-L27), and RT-PCR assays for GIV-L1and GI-L1 confirmed the presence of multiple IBV genotypes and lineages in 6 of the 9 IBV-positive samples (#1, #2, #.....
    Document: Sequencing data was further analyzed to determine if multiple IBV genotypes or lineages could be detected. Lineage-1 and lineage-27 from Genotype I (GI-L1, GI-L27), and lineage-1 from Genotype IV (GIV-L1) were the detected lineages in the samples. Results from individual RT-qPCR assay for GA08 (GI-L27), and RT-PCR assays for GIV-L1and GI-L1 confirmed the presence of multiple IBV genotypes and lineages in 6 of the 9 IBV-positive samples (#1, #2, #3, #4, #6, #7) and AmpSeq results confirmed multiple genotypes and lineages in 4 (#2, #4, #6, #7) of those 6 samples (AmpSeq failed to detect any IBV in #1, and failed to detect GIV-L1 in #3). All rights reserved. No reuse allowed without permission.

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