Author: Alisha Chitrakar; Sneha Rath; Jesse Donovan; Kaitlin Demarest; Yize Li; Raghavendra Rao Sridhar; Susan R. Weiss; Sergei V. Kotenko; Ned S. Wingreen; Alexei Korennykh
Title: Realtime 2-5A kinetics suggests interferons ß and ? evade global arrest of translation by RNase L Document date: 2018_11_26
ID: mxdvdw9u_32
Snippet: Treatments of naive cells with conditioned media were done in a separate 12well plate and using cells seeded 1 day before the analysis. Naïve cell media was replaced with 1 mL of the conditioned medium from above. Cells were grown in the conditioned media for 16 hours and harvested in 300 μL RLT (Qiagen). For pulse-chase experiments in Fig. 5C , 10 μM anisomycin was added 3 hours after poly-IC addition......
Document: Treatments of naive cells with conditioned media were done in a separate 12well plate and using cells seeded 1 day before the analysis. Naïve cell media was replaced with 1 mL of the conditioned medium from above. Cells were grown in the conditioned media for 16 hours and harvested in 300 μL RLT (Qiagen). For pulse-chase experiments in Fig. 5C , 10 μM anisomycin was added 3 hours after poly-IC addition.
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