Author: Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; Christos Spanos; Juri Rappsilber; Alfredo Castello; Gracjan Michlewski
Title: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination Document date: 2017_10_9
ID: ifla4aix_36
Snippet: To test if RNA is necessary for efficient TRIM25 ligase activity, we performed in vitro ubiquitination assays with various TRIM25 constructs (Fig. 6a) . Incubation of T7-TRIM25 or T7-TRIM25ΔRBD IPed from HeLa cells with recombinant proteins from the ubiquitin pathway (Ube2D3, UBE1, and Ubiquitin) revealed efficient, time-dependent ubiquitination of All rights reserved. No reuse allowed without permission......
Document: To test if RNA is necessary for efficient TRIM25 ligase activity, we performed in vitro ubiquitination assays with various TRIM25 constructs (Fig. 6a) . Incubation of T7-TRIM25 or T7-TRIM25ΔRBD IPed from HeLa cells with recombinant proteins from the ubiquitin pathway (Ube2D3, UBE1, and Ubiquitin) revealed efficient, time-dependent ubiquitination of All rights reserved. No reuse allowed without permission.
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