Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells Document date: 2020_4_9
ID: fvjh3g7v_18
Snippet: To determine the optimal incubation period for detection of VSVdG-SARS-CoV-2-Sdel18 infection, a time-course monitoring of EGFP expression was performed using Opera Phenix High Content Screening System. EGFP expression could be detected as early as 8 hours post infection, and the number of GFP-positive cells gradually increased, reaching the plateau phase within 12 hours, and remained stable between 12-24 hours ( Figure 4 ). Whereas the fluoresce.....
Document: To determine the optimal incubation period for detection of VSVdG-SARS-CoV-2-Sdel18 infection, a time-course monitoring of EGFP expression was performed using Opera Phenix High Content Screening System. EGFP expression could be detected as early as 8 hours post infection, and the number of GFP-positive cells gradually increased, reaching the plateau phase within 12 hours, and remained stable between 12-24 hours ( Figure 4 ). Whereas the fluorescence intensity was increased continuously. As pseudoviruses are unable to produce progeny viruses, the infected cell number would be a better indicator for infection efficiency. Therefore, the infection efficiency could be quantitative evaluated at 12-24 hours by counting the number of GFP-positive cells.
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