Author: Lu Guo; Xuehan Sun; Xinge Wang; Chen Liang; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Liu Wang; Ruiqi Rachel Wang; Qi Zhou; Wei Li
Title: SARS-CoV-2 detection with CRISPR diagnostics Document date: 2020_4_11
ID: 2una9767_9
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.10.023358 doi: bioRxiv preprint GISAID, 1673 of them contained sequences matching our chosen primers and sgRNA. Only 3 of them have 1 mismatch to the forward primer and only 2 of them have 1 mismatch to the reverse primer (Supplementary information, Fig. S5 ), suggesting that our selected sgRNA and primers can be used for nearly all of th.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.10.023358 doi: bioRxiv preprint GISAID, 1673 of them contained sequences matching our chosen primers and sgRNA. Only 3 of them have 1 mismatch to the forward primer and only 2 of them have 1 mismatch to the reverse primer (Supplementary information, Fig. S5 ), suggesting that our selected sgRNA and primers can be used for nearly all of the reported SARS-CoV-2 genomes. Meanwhile, we aligned 12 typical human coronaviruses, and found that none of the whole set of primers and sgRNA showed high similarity (Supplementary information, Fig. S6a-c) .
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