Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein Document date: 2020_3_14
ID: 5hpbjkft_28
Snippet: ELISA assay was performed to identify the interaction of CD147 and SP. The His-SP (RBD) protein was coated on microplate, and then incubated with different concentrations of His-CD147 protein (2-fold dilution, 400-0.195 μg/mL) at 37°C for 1 hour. After washing with PBST, the samples were incubated with anti-CD147 antibody (HAb18, produced by our laboratory) for 1 hour; and then incubated with horseradish peroxidase (HRP)-labeled goat anti-mouse.....
Document: ELISA assay was performed to identify the interaction of CD147 and SP. The His-SP (RBD) protein was coated on microplate, and then incubated with different concentrations of His-CD147 protein (2-fold dilution, 400-0.195 μg/mL) at 37°C for 1 hour. After washing with PBST, the samples were incubated with anti-CD147 antibody (HAb18, produced by our laboratory) for 1 hour; and then incubated with horseradish peroxidase (HRP)-labeled goat anti-mouse antibody for 1 hour. After coloration, the OD value at 450 nm was measured with a full-wavelength microplate reader (Epoch,
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