Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein Document date: 2020_3_14
ID: 5hpbjkft_8
Snippet: The antiviral tests were performed to investigate the possible function of CD147 in SARS-CoV-2 invasion for host cells. Vero E6 cells were cultured in a 96-well plate, and CD147 on Vero E6 cells was blocked with Meplazumab. Then, the cells were infected with SARS-CoV-2 (100TCID 50 ) and the cell damage of Vero E6 and viral load were detected by crystal violet staining and quantitative PCR, respectively. Compared with the control group, the inhibi.....
Document: The antiviral tests were performed to investigate the possible function of CD147 in SARS-CoV-2 invasion for host cells. Vero E6 cells were cultured in a 96-well plate, and CD147 on Vero E6 cells was blocked with Meplazumab. Then, the cells were infected with SARS-CoV-2 (100TCID 50 ) and the cell damage of Vero E6 and viral load were detected by crystal violet staining and quantitative PCR, respectively. Compared with the control group, the inhibitory rate for viruses was significantly increased in CD147-blocking group in a dose-dependent manner, with a concentration for 50% of maximal effect (EC 50 ) of 24.86 μg/mL ( Figure 1A ) and the half maximal inhibitory concentration (IC 50 ) of 15.16 μg/mL ( Figure 1B) . These results show that blocking CD147 on the host cells has an inhibitory effect on SARS-CoV-2, suggesting an essential role of CD147 in facilitating SARS-CoV-2 invasion for host cells.
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