Scrutinizing the SARS-CoV-2 protein information for the designing an effective vaccine encompassing both the T-cell and B-cell epitopes

Neha Jain; Uma Shankar; Prativa Majee; Amit Kumar

Analysis of the physiochemical properties of a multi-subunit vaccine helps in analyzing the optimal immune response of the vaccine and its stability inside the host. ProtParam was utilized for accessing the molecular weight, pI, stability and grand average of hydropathy (GRAVY) constant of the SARS-CoV-2 multi-epitope vaccine construct. Molecular weight . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.26.009209 doi: bioRxiv preprint of the vaccine construct was observed to be 65.3 kDa while pI value was 10.06. Instability index of 31.93 (<40) depicted the stable nature of the vaccine construct while the estimated half-life in mammalian reticulocytes was observed to be >30 hours. GRAVY constant of 0.213 depicted the hydrophilicity of the vaccine construct and was predicted to be soluble with a probability of `70 % as predicted by SolPro (Table 2) . Antigenicity is a preliminary requisite of a successful vaccine candidate. A vaccine construct must possess both immunogenicity and antigenicity to elicit the humoral and cell-mediated immune response.