Selected article for: "Escherichia coli and expression vector"
Author: Neha Jain; Uma Shankar; Prativa Majee; Amit Kumar
Title: Scrutinizing the SARS-CoV-2 protein information for the designing an effective vaccine encompassing both the T-cell and B-cell epitopes
  • Document date: 2020_4_1
    • ID: lmstdmyb_70
    Snippet: Escherichia coli K12 strain was selected as a host for cloning purpose as the expression and purification of multi-epitope vaccines are easier in this bacterium. pET28a(+) expression vector was cleaved using BamHI and HindIII restriction enzyme and the cDNA was inserted near the ribosome binding site using Snapgene. 6X histidine tag was added at the 3' end for the isolation and purification of the vaccine construct ( Figure 9C )......
    Document: Escherichia coli K12 strain was selected as a host for cloning purpose as the expression and purification of multi-epitope vaccines are easier in this bacterium. pET28a(+) expression vector was cleaved using BamHI and HindIII restriction enzyme and the cDNA was inserted near the ribosome binding site using Snapgene. 6X histidine tag was added at the 3' end for the isolation and purification of the vaccine construct ( Figure 9C ).

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