Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs Document date: 2020_3_9
ID: 1uqjmeic_30
Snippet: The probe design is unique in that two DNA probes are designed to expose single-292 stranded target recognition parts, enabling hybridization of the target RNA and the probes at 293 37℃. The hybridization sequences were systematically selected using the nucleic acid design 294 software Primer-BLAST and NUPACK to minimize any structure formation while 295 maximizing hybridization to the target RNA. The efficient hybridization between the probes .....
Document: The probe design is unique in that two DNA probes are designed to expose single-292 stranded target recognition parts, enabling hybridization of the target RNA and the probes at 293 37℃. The hybridization sequences were systematically selected using the nucleic acid design 294 software Primer-BLAST and NUPACK to minimize any structure formation while 295 maximizing hybridization to the target RNA. The efficient hybridization between the probes 296 and target RNA is one of the reasons for enabling high sensitivity during the isothermal 297
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