Selected article for: "absence presence and gene activity"
Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2
  • Document date: 2018_11_29
    • ID: ccfrx3md_5
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/483289 doi: bioRxiv preprint levels were influenced by the presence or absence of serum implied that regulation was 236 either at the level of the gene, the protein or both. To address whether or not serum had an 237 effect on altering the activity of the PARP2 gene, and specifically the SRE in its promoter, 238 MCF-7 cells were cu.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/483289 doi: bioRxiv preprint levels were influenced by the presence or absence of serum implied that regulation was 236 either at the level of the gene, the protein or both. To address whether or not serum had an 237 effect on altering the activity of the PARP2 gene, and specifically the SRE in its promoter, 238 MCF-7 cells were cultured in CM or SF medium for 30 minutes and then total RNA was 239 extracted and used for cDNA synthesis. Endpoint and quantitative RT-PCR using PARP2 240 primers showed that PARP2 mRNA was available regardless of the treatment (Fig. 1C, D) . 241

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