Author: Lyudmila Kovalchuke; Eugene V. Mosharov; Oren A. Levy; Lloyd A. Greene
Title: Stress-induced phospho-ubiquitin formation causes parkin degradation Document date: 2018_12_5
ID: ceepyyxj_13
Snippet: The kinase PINK1 has been reported to play a role in parkin turnover, including that caused by hydrogen peroxide exposure [41] , [67] . We therefore examined whether PINK1 is involved in L-DOPA-dependent parkin loss. To do this, we knocked down PINK1 in differentiated PC12 cells using a previously-characterized shRNA [68] . PINK1 knockdown was very effective at the mRNA level, reducing PINK1 transcript levels to 14.1 ± 3.5% of control levels aft.....
Document: The kinase PINK1 has been reported to play a role in parkin turnover, including that caused by hydrogen peroxide exposure [41] , [67] . We therefore examined whether PINK1 is involved in L-DOPA-dependent parkin loss. To do this, we knocked down PINK1 in differentiated PC12 cells using a previously-characterized shRNA [68] . PINK1 knockdown was very effective at the mRNA level, reducing PINK1 transcript levels to 14.1 ± 3.5% of control levels after 4 days (p = 0.0001, N = 4) (Fig. 3A) .
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