Author: Lyudmila Kovalchuke; Eugene V. Mosharov; Oren A. Levy; Lloyd A. Greene
Title: Stress-induced phospho-ubiquitin formation causes parkin degradation Document date: 2018_12_5
ID: ceepyyxj_76
Snippet: Early passage 293T cells were plated at a density of ~14-18 x 10 6 cells/15cm plate. The next day, cells were transfected with 21 μg expression vector, 16.5 μg psPAX2, and 7.5 μg VSVg plasmids per plate using the calcium phosphate transfection method. Virus-containing medium was harvested 2 and 3 days after transfection, pooled, passed through a 0.45 μm filter, 3% (w/v) hydrogen peroxide was diluted with sterile distilled water 1:1000 before .....
Document: Early passage 293T cells were plated at a density of ~14-18 x 10 6 cells/15cm plate. The next day, cells were transfected with 21 μg expression vector, 16.5 μg psPAX2, and 7.5 μg VSVg plasmids per plate using the calcium phosphate transfection method. Virus-containing medium was harvested 2 and 3 days after transfection, pooled, passed through a 0.45 μm filter, 3% (w/v) hydrogen peroxide was diluted with sterile distilled water 1:1000 before subsequent dilution in fresh medium.
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