Author: Anniina Vihervaara; Dig Bijay Mahat; Samu V. Himanen; Malin A.H. Blom; John T. Lis; Lea Sistonen
Title: Stress-Induced Transcriptional Memory Accelerates Promoter-Proximal Pause-Release and Decelerates Termination over Mitotic Divisions Document date: 2019_3_14
ID: i3owd6b0_47
Snippet: To call significant changes in gene and enhancer transcription, we utilized DESeq2 (Love et 571! al., 2014) , which uses the variance in biological replicates to assess significant changes 572! between conditions. Differential gene expression was quantified using gene body 573! Figure S5B ), conducting DESeq2 using the same time point from unconditioned and 590! preconditioned cells as a replicate pair. These analyses showed 227 genes and 496 enh.....
Document: To call significant changes in gene and enhancer transcription, we utilized DESeq2 (Love et 571! al., 2014) , which uses the variance in biological replicates to assess significant changes 572! between conditions. Differential gene expression was quantified using gene body 573! Figure S5B ), conducting DESeq2 using the same time point from unconditioned and 590! preconditioned cells as a replicate pair. These analyses showed 227 genes and 496 enhancers 591! to be HSF1 dependent in both unconditioned and preconditioned K562 cells ( Figure S4B) .
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