Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow Document date: 2020_3_10
ID: kv77pw7y_24
Snippet: The sensitivities observed in this study were slightly different than the described for RdRP (3.6 copies per reaction) and E (3.9 copies per reaction) original description, where the authors used the in vitro transcribed SARS-CoV-2 RNA directly in the reaction 3 . Our results can be secondary to the fact that we spiked the synthetic RNA in the nasopharyngeal samples, resembling a real clinical sample and in the limit of detection calculation, we .....
Document: The sensitivities observed in this study were slightly different than the described for RdRP (3.6 copies per reaction) and E (3.9 copies per reaction) original description, where the authors used the in vitro transcribed SARS-CoV-2 RNA directly in the reaction 3 . Our results can be secondary to the fact that we spiked the synthetic RNA in the nasopharyngeal samples, resembling a real clinical sample and in the limit of detection calculation, we assumed that the nucleic acid purification recovered 100% of the spiked RNA sequences of the 200 ul aliquot used in the extraction.
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