Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays Document date: 2020_4_1
ID: 6mdimxnk_26
Snippet: Our comparative results of primer-probe sets used in qRT-PCR assays indicate some variation in the analytical sensitivities for SARS-CoV-2 detection and ability to differentiate between true negatives and positives at low RNA concentrations. We found that the most sensitive primer-probe sets are E-Sarbeco (Charité), HKU-ORF1 (HKU), HKU-N (HKU), and 2019-nCoV_N1 (US CDC), which could partially (63-75%) detect SARS-CoV-2 at 10 virus copies per μL.....
Document: Our comparative results of primer-probe sets used in qRT-PCR assays indicate some variation in the analytical sensitivities for SARS-CoV-2 detection and ability to differentiate between true negatives and positives at low RNA concentrations. We found that the most sensitive primer-probe sets are E-Sarbeco (Charité), HKU-ORF1 (HKU), HKU-N (HKU), and 2019-nCoV_N1 (US CDC), which could partially (63-75%) detect SARS-CoV-2 at 10 virus copies per μL of RNA. In contrast, the RdRp-SARSr (Charité) primer-probe set had the lowest sensitivity, as also shown by an independent study 13 , likely stemming from a mismatch in the reverse primer. Furthermore, we found background cross-reactivity when testing nasopharyngeal swabs prior to the COVID-19 pandemic using the China CDC and US CDC assays. While the China CDC "N" and "ORF1" primer-probe sets require further investigation, we found that the background cross-reactivity did not affect the outcomes of the US CDC assay when testing clinical samples from the COVID-19 pandemic. Overall, our findings indicate that the qRT-PCR assay from HKU, the screening assay (E-Sarbeco) from Charité, and the updated assay from the US CDC are most reliable for accurate detection of SARS-CoV-2.
Search related documents:
Co phrase search for related documents- accurate detection and CDC assay: 1, 2, 3, 4
- accurate detection and China CDC: 1, 2
- accurate detection and clinical sample: 1, 2, 3, 4, 5, 6, 7, 8
- accurate detection and cross reactivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- accurate detection and low sensitivity: 1, 2, 3, 4, 5, 6
- accurate detection and nasopharyngeal swab: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- accurate detection and nasopharyngeal swab test: 1, 2, 3, 4, 5
- accurate detection and PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52
- accurate detection and positive true negative: 1
- accurate detection and primer probe: 1, 2, 3, 4, 5, 6, 7
- accurate detection and primer probe set: 1
- accurate detection and qrt PCR assay: 1, 2, 3
- accurate detection and RNA concentration: 1, 2, 3
- accurate detection and SARS accurate detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- accurate detection and SARS detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- accurate detection and screening assay: 1, 2, 3, 4, 5, 6, 7
- accurate detection and true negative: 1, 2, 3, 4, 5
- accurate detection and virus copy: 1, 2
- analytical sensitivity and CDC assay: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date