Author: Lulin Zhou; Zubiao Niu; Xiaoyi Jiang; Zhengrong Zhang; You Zheng; Zhongyi Wang; Yichao Zhu; Lihua Gao; Xiaoning Wang; Qiang Sun
Title: Systemic analysis of tissue cells potentially vulnerable to SARS-CoV-2 infection by the protein-proofed single-cell RNA profiling of ACE2, TMPRSS2 and Furin proteases Document date: 2020_4_10
ID: 3btc31kj_14
Snippet: We applied a graph-based clustering approach to assemble cells. Briefly, we constructed a K-nearest neighbour graph based on the Euclidean distance in principal component analysis(PCA) space and refined the edge weights between any two cells based on the shared overlap in their local neighbourhoods through the "Find Neighbors" function in Seurat. Subsequently, we applied the Louvain algorithm using the "Find Cluster" function in Seurat to group c.....
Document: We applied a graph-based clustering approach to assemble cells. Briefly, we constructed a K-nearest neighbour graph based on the Euclidean distance in principal component analysis(PCA) space and refined the edge weights between any two cells based on the shared overlap in their local neighbourhoods through the "Find Neighbors" function in Seurat. Subsequently, we applied the Louvain algorithm using the "Find Cluster" function in Seurat to group cells.
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