Selected article for: "PCR analysis and positive control"
Author: Lea Gaucherand; Brittany K. Porter; Summer K. Schmaling; Christopher Harley Rycroft; Yuzo Kevorkian; Craig McCormick; Denys A. Khaperskyy; Marta Maria Gaglia
Title: The influenza A virus endoribonuclease PA-X usurps host mRNA processing machinery to limit host gene expression
  • Document date: 2018_10_14
    • ID: 8k7w467p_7
    Snippet: We have previously shown that PA-X preferentially degrades RNA transcribed by Pol II 197 (Khaperskyy et al., 2016) . A key difference between transcripts of Pol II and other cellular Pols 198 is that Pol II RNAs can be spliced. The process of splicing is mechanistically linked to 199 IAV PA(ΔX) virus ( Figure S4A ), suggesting that PA-X down-regulates IFN-λ2 mRNA in 246 infected cells. We co-transfected the IFN-l2 expression vectors with PR8 PA.....
    Document: We have previously shown that PA-X preferentially degrades RNA transcribed by Pol II 197 (Khaperskyy et al., 2016) . A key difference between transcripts of Pol II and other cellular Pols 198 is that Pol II RNAs can be spliced. The process of splicing is mechanistically linked to 199 IAV PA(ΔX) virus ( Figure S4A ), suggesting that PA-X down-regulates IFN-λ2 mRNA in 246 infected cells. We co-transfected the IFN-l2 expression vectors with PR8 PA-X and a luciferase 247 construct containing an intron (Younis et al., 2010), which served as a positive control for PA-X 248 activity. We confirmed that IFN-λ2 mRNA was expressed and exported into the cytoplasm at 249 similar levels irrespective of the construct used ( Figures S4B, S4C) . We also checked that the 250 five introns were properly spliced by PCR analysis across the splice sites ( Figures 5E, S4D) . 251

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