Selected article for: "AccuPlex SARS reference material and SARS reference"
Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step
  • Document date: 2020_4_8
    • ID: ka3n9pft_8
    Snippet: . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.028811 doi: bioRxiv preprint Then, 100 µL of AccuPlex SARS-CoV-2 reference material was processed in Promega Maxwell device (using an AS1520 cartridge) and eluted in 100 µL of elution buffer. The extracted template (4 µL) was added to master mix, and RT.....
    Document: . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.028811 doi: bioRxiv preprint Then, 100 µL of AccuPlex SARS-CoV-2 reference material was processed in Promega Maxwell device (using an AS1520 cartridge) and eluted in 100 µL of elution buffer. The extracted template (4 µL) was added to master mix, and RT-qPCR amplification was carried out in a Bio-Rad (CFX-96) thermal cycler along with the reactions with unprocessed samples.

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