The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV)

Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa

In this context, the Y motif may have other functions such as acting as an intracellular retention signal or maintaining correct protein conformation. In animal viruses for instance, Y motifs in viral glycoproteins have been reported to function as intracellular retention signals (reviewed in Ujike and Taguchi et al., 2015) . In PSLV and PMTV, the Y motif is required for the delivery of viral proteins to the cell periphery and PD, but this motif is important for conformation of the protein, rather than . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint its internalization. In fact, membrane topology analysis of PMTV TGB3 revealed that YQDLN motif resides in ER lumen (Tilsner et al., 2010) . We previously identified three amino acid residues (98, 150 and 169) within the core region of OuMV MP (63-206), which are responsible for localization of MP at the cell periphery, and these residues are possibly involved in the correct folding/function of OuMV MP (Margaria et al., 2016) .