Selected article for: "discontinuous RNA synthesis and leader sequence"
Author: Hazel Stewart; Katherine Brown; Adam M. Dinan; Nerea Irigoyen; Eric J. Snijder; Andrew E. Firth
Title: The transcriptional and translational landscape of equine torovirus
  • Document date: 2018_4_7
    • ID: mozfm5ds_21
    Snippet: Three chimaeric junctions were identified where the first nucleotide of the 227 corresponding read mapped to the first nucleotide of the viral genome. Of these, 228 one junction was consistent with the previously characterised sgRNA produced via 229 discontinuous RNA synthesis encoding the S gene (280 reads, or 3 % of total 230 chimaeric reads) (12). These reads spanned the entire leader-body junction of the S 231 gene, possessing 14 -18 nt of th.....
    Document: Three chimaeric junctions were identified where the first nucleotide of the 227 corresponding read mapped to the first nucleotide of the viral genome. Of these, 228 one junction was consistent with the previously characterised sgRNA produced via 229 discontinuous RNA synthesis encoding the S gene (280 reads, or 3 % of total 230 chimaeric reads) (12). These reads spanned the entire leader-body junction of the S 231 gene, possessing 14 -18 nt of the 5' UTR (i.e. the actual 5'-derived sequence is at 232 least 14 nt, ACGUAUCUUUAGAA, comprising the so-called 6-nt leader, the leader TRS 233 CUUUAGA, and an additional A), followed by the stretch of ORF1b just upstream of 234 the S gene. A second set of transcripts containing 5' leader sequence was identified 235 by four unique reads starting with the 5' leader (ACGUAU) and TRS sequence 236 (CUUUAGA), where the remainder of the read mapped to the start of the N gene. 237

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