Author: Hazel Stewart; Katherine Brown; Adam M. Dinan; Nerea Irigoyen; Eric J. Snijder; Andrew E. Firth
Title: The transcriptional and translational landscape of equine torovirus Document date: 2018_4_7
ID: mozfm5ds_28
Snippet: This Ribo-seq data confirmed translation of two ORFs located within the so-called 5' 294 UTR and overlapping the 5' end of ORF1a. We have termed these U1 (80 codons) and 295 U2 (258 codon). We predict that translation of both U1 and U2 is initiated from CUG 296 codons, as a close inspection indicated that ribosomes accumulated at these two 297 sites (Figure 7 ). It must be noted that pretreatment with CHX or HAR can introduce 298 artefacts into r.....
Document: This Ribo-seq data confirmed translation of two ORFs located within the so-called 5' 294 UTR and overlapping the 5' end of ORF1a. We have termed these U1 (80 codons) and 295 U2 (258 codon). We predict that translation of both U1 and U2 is initiated from CUG 296 codons, as a close inspection indicated that ribosomes accumulated at these two 297 sites (Figure 7 ). It must be noted that pretreatment with CHX or HAR can introduce 298 artefacts into ribosome profiling data: CHX can lead to an excess of RPF density over 299 ~30 codons following initiation sites when cells are stressed (15, 20) . It has also been 300 suggested that both drugs can promote upstream initiation due to scanning pre-301 initiation complexes stacking behind ribosomes paused at canonical initiation sites 302 (21). However, the distance between the U1 CUG, the U2 CUG and the ORF1a 303 initiation site, besides observation of efficient translation of U2 downstream of the 304
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