Selected article for: "current study and gene expression"
Author: Hazel Stewart; Katherine Brown; Adam M. Dinan; Nerea Irigoyen; Eric J. Snijder; Andrew E. Firth
Title: The transcriptional and translational landscape of equine torovirus
  • Document date: 2018_4_7
    • ID: mozfm5ds_52
    Snippet: To the best of our knowledge, this is the first analysis of differential gene expression What is the function of U1 and U2? The current lack of a published reverse genetics 478 system to study torovirus replication means we are unable to perform targeted 479 mutagenesis. This would enable definitive experimental confirmation that U1 and U2 480 are translated from their respective CUG codons, followed by phenotypic analysis of 481 knock-out mutant.....
    Document: To the best of our knowledge, this is the first analysis of differential gene expression What is the function of U1 and U2? The current lack of a published reverse genetics 478 system to study torovirus replication means we are unable to perform targeted 479 mutagenesis. This would enable definitive experimental confirmation that U1 and U2 480 are translated from their respective CUG codons, followed by phenotypic analysis of 481 knock-out mutants. However the comparative genomic analysis together with the 482 accumulation of ribosomes on both CUG codons is highly suggestive of this being the 483 site of initiation; CUG has previously been reported as the most commonly utilised 484 non-AUG initiation codon in mammalian systems (43). In the case of U1, the coding 485 sequence contains no AUG codons (in any frame), a situation that would facilitate 486 pre-initiation ribosomes to continue scanning to the U2 CUG and the ORF1a AUG 487 initiation sites (44). It remains a possibility that U2 translation initiates at a 488 downstream AUG, however the only in-frame AUG is located 336 nt downstream of 489 our presumed start site and is in a poor initiation context ('C' at −3) and 3' of the 490

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