Author: Sean Ekins; Peter B. Madrid
Title: Tilorone: A Broad-Spectrum Antiviral For Emerging Viruses Document date: 2020_3_11
ID: 2suz20me_5
Snippet: Cells were maintained in MEM or DMEM supplemented with FBS as required for each 57 cell line. For antiviral assays the same medium was used but with FBS reduced to 2% 58 or less and supplemented with 50 µg/ml gentamicin. The test compound is prepared at 59 four log 10 final concentrations 0.1, 1.0, 10, and 100 µg/ml or µM. Five microwells are 60 used per dilution: three for infected cultures and two for uninfected toxicity cultures. 127 . CC-B.....
Document: Cells were maintained in MEM or DMEM supplemented with FBS as required for each 57 cell line. For antiviral assays the same medium was used but with FBS reduced to 2% 58 or less and supplemented with 50 µg/ml gentamicin. The test compound is prepared at 59 four log 10 final concentrations 0.1, 1.0, 10, and 100 µg/ml or µM. Five microwells are 60 used per dilution: three for infected cultures and two for uninfected toxicity cultures. 127 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.09.984856 doi: bioRxiv preprint
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