Under-the-radar dengue virus infections in natural populations of Aedes aegypti mosquitoes

Sean M. Boyles; Carla N. Mavian; Esteban Finol; Maria Ukhanova; Caroline J. Stephenson; Gabriela Hamerlinck; Seokyoung Kang; Caleb Baumgartner; Mary Geesey; Israel Stinton; Kate Williams; Derrick K. Mathias; Mattia Prosperi; Volker Mai; Marco Salemi; Eva A. Buckner; John A. Lednicky; Adam R. Rivers; Rhoel R. Dinglasan

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.24.919282 doi: bioRxiv preprint The proportion of the sub-composition is summarized at the species level for most viruses; however, some viruses were classified at higher levels if species could not be determined by the lowest common ancestor method. 1) . The analysis was conducted utilizing full genome coding sequences in JCoDA using a sliding window analysis with a window size of 10. A small genome schematic is placed below the graph to its scale. Line colors approaching red from orange lie at higher values on the graph to indicate highe dN/dS values. (b) Using all available sequences, a dN/dS comparison was conducted to calculate mean ratio values within DENV4 overall, DENV4 genotype II, DENV4 genotype IIb, DENV4 FL-American-Caribbean clades, and DENV4-Florida-Caribbean-specific clades (respectively moving along Nodes C, B, and A from fig. 3B ). Adjacent to the IIb-containing comparison, a comparison between all available DENV4 genome sequences, DENV4 genotype II, and DENV4 genotype IIa is depicted. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.24.919282 doi: bioRxiv preprint