Selected article for: "FPLC gel filtration chromatography purification and gel filtration"

Author: Amrita Roy; Liangzhong Lim; Shagun Srivastava; Jianxing Song
Title: Unique properties of Zika NS2B-NS3pro complexes as decoded by experiments and MD simulations
  • Document date: 2016_9_28
  • ID: l82aakrk_11
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/078113 doi: bioRxiv preprint Catalysis, inhibition and dynamics of Zika NS2B-NS3pro 6 precipitation (column 2 of Fig. 1D ). Subsequently, linked NS2B-NS3pro without His-tag (column 3 of Fig. 1D ) was obtained by the cleavage with thrombin covalently linked to beads, followed by binding to Ni 2+ -beads and final purification with FPLC gel-filtrati.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/078113 doi: bioRxiv preprint Catalysis, inhibition and dynamics of Zika NS2B-NS3pro 6 precipitation (column 2 of Fig. 1D ). Subsequently, linked NS2B-NS3pro without His-tag (column 3 of Fig. 1D ) was obtained by the cleavage with thrombin covalently linked to beads, followed by binding to Ni 2+ -beads and final purification with FPLC gel-filtration chromatography as described above.

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