Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_20
Snippet: In this pilot experiment, we performed a initial limit of detection using two separate synthetic RNA SARS-CoV-2 sources (BEI NR-52358 and ATCC VR3276T) with a 2-fold dilution series ranging from approximately 100 viral copies per microliter (cp/µL) to 3 cp/µL. The assay successfully detected both samples down to a calculated lower boundary of 2.8 cp/µL with maximum observed Ct values of 36.19 (Table 4 )......
Document: In this pilot experiment, we performed a initial limit of detection using two separate synthetic RNA SARS-CoV-2 sources (BEI NR-52358 and ATCC VR3276T) with a 2-fold dilution series ranging from approximately 100 viral copies per microliter (cp/µL) to 3 cp/µL. The assay successfully detected both samples down to a calculated lower boundary of 2.8 cp/µL with maximum observed Ct values of 36.19 (Table 4 ).
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