Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_18
Snippet: Detection of targets was compared for crossing point values (Cp) for the four prepared buffers and the Roche external lysis buffer as shown in Table 1 . Automated extraction was conducted using the Roche MagNa pure LC and the PCR was performed on the Roche z480 RT PCR instrument. On a second day, the 4 M GITC buffer was tested in comparison with Roche and Qiagen buffers, using the IndiMag48 extraction system and RT PCR was conducted on a Roche Li.....
Document: Detection of targets was compared for crossing point values (Cp) for the four prepared buffers and the Roche external lysis buffer as shown in Table 1 . Automated extraction was conducted using the Roche MagNa pure LC and the PCR was performed on the Roche z480 RT PCR instrument. On a second day, the 4 M GITC buffer was tested in comparison with Roche and Qiagen buffers, using the IndiMag48 extraction system and RT PCR was conducted on a Roche LightCycler II 480 ( Table 2) . On the basis of these combined data, the buffer containing 4 M GITC was confirmed as suitable for use with the diagnostic systems in CUH. Figure 1 shows the relative performance of all buffers tested, measured using Cp values The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.05.026435 doi: bioRxiv preprint
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