Author: Chuang Liu; Yang Yang; Yuanzhu Gao; Chenguang Shen; Bin Ju; Congcong Liu; Xian Tang; Jinli Wei; Xiaomin Ma; Weilong Liu; Shuman Xu; Yingxia Liu; Jing Yuan; Jing Wu; Zheng Liu; Zheng Zhang; Peiyi Wang; Lei Liu
Title: Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM Document date: 2020_3_5
ID: cr22vp8b_16
Snippet: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.02.972927 doi: bioRxiv preprint laboratory. BALF sample was centrifuged at 5, 000 rpm at 4℃ for 5 minutes, and then 200 µl of the supernatant was added to the monolayer of cell and incubated at 37°C and 5% CO 2 for 1 hour. Then the cells were washed wi.....
Document: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.02.972927 doi: bioRxiv preprint laboratory. BALF sample was centrifuged at 5, 000 rpm at 4℃ for 5 minutes, and then 200 µl of the supernatant was added to the monolayer of cell and incubated at 37°C and 5% CO 2 for 1 hour. Then the cells were washed with PBS for 3 times, and the fresh DMEM containing 2% fetal bovine serum (FBS) and 1% penicillin streptomycin (PS) was added to the cell culture. Cells were maintained at 37°C and 5% CO 2 , and CPEs were monitored daily with light microscopy. Meanwhile viral RNAs were detected at 3 and 5 dpi using RT-PCR to monitor the replication of virus.
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